The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 21, 2022
Filed:
Nov. 14, 2016
Arizona Board of Regents on Behalf of Arizona State University, Scottsdale, AZ (US);
Deirdre Meldrum, Phoenix, AZ (US);
Laimonas Kelbauskas, Gilbert, AZ (US);
Wacey Teller, Mesa, AZ (US);
Meryl Rodrigues, Tempe, AZ (US);
Hong Wang, Tempe, AZ (US);
Ganquan Song, Mesa, AZ (US);
Yanqing Tian, Tempe, AZ (US);
Fengyu Su, Tempe, AZ (US);
Xiangxing Kong, Tempe, AZ (US);
Liqiang Zhang, Chandler, AZ (US);
Arizona Board of Regents on Behalf of Arizona State University, Scottsdale, AZ (US);
Abstract
A microfluidic device includes a first substrate including at least one microfluidic channel and a plurality of microwells, as well as a cooperating second substrate defining multiple split-walled cell trap structures that are registered with and disposed within the plurality of microwells. A method for performing an assay includes flowing cells and a first aqueous medium into a plurality of microwells of a microfluidic device, wherein each microwell includes a cell trap structure configured to trap at least one cell. The method further comprises flowing a nonpolar fluid with low permeability for analytes of interest through a microfluidic channel to flush a portion of the first aqueous medium from the microfluidic channel while retaining another portion of the first aqueous medium and at least one cell within each microwell. Surface tension at a non-polar/polar medium interface prevents molecule exchange between interior and exterior portions of microwells.