The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 21, 2022

Filed:

Apr. 08, 2016
Applicant:

Medical Research Council, Swindon, GB;

Inventors:

Anne Bertolotti, Cambridge, GB;

Indrajit Das, Cambridge, GB;

Agnieszka Krzyzosiak, Cambridge, GB;

Adrien Rousseau, Cambridge, GB;

Kim Schneider, Cambridge, GB;

Anna Gudny Sigurdardottir, Cambridge, GB;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
A61K 31/155 (2006.01); C07C 281/18 (2006.01); C07D 213/61 (2006.01); A61P 21/00 (2006.01); A61P 25/14 (2006.01); C12Q 1/42 (2006.01); C07D 239/30 (2006.01); A61P 25/28 (2006.01); G01N 33/573 (2006.01); C12N 11/14 (2006.01); G01N 21/31 (2006.01); G01N 33/53 (2006.01);
U.S. Cl.
CPC ...
A61K 31/155 (2013.01); A61P 21/00 (2018.01); A61P 25/14 (2018.01); A61P 25/28 (2018.01); C07C 281/18 (2013.01); C07D 213/61 (2013.01); C07D 239/30 (2013.01); C12N 11/14 (2013.01); C12Q 1/42 (2013.01); G01N 21/31 (2013.01); G01N 33/53 (2013.01); G01N 33/573 (2013.01); C07K 2317/92 (2013.01); G01N 2333/916 (2013.01);
Abstract

The present invention discloses a method to discover selective inhibitors of phosphatases. Thus the invention provides a method for screening a test compound to determine whether the compound binds a holophosphatase selectively or non-selectively comprising: i) providing a first holophosphatase wherein said holophosphatase is captured/immobilised; ii) testing a test compound for its ability to bind to the first holophosphatase; iii) providing a second holophosphatase wherein said second holophosphatase is captured/immobilised; iv) testing the same test compound for its ability to bind to the second holophosphatase; v) comparing the binding of the test compound to said first holophosphatase with the binding to said second phosphatase wherein a compound that binds a holophosphatase selectively will bind to said first holophosphatase but not said second holophosphatase; or will bind to said second holophosphatase but not said first; or wherein a compound that binds a holophosphatase non-selectively will bind to both said first holophosphatase and said second holophosphatase.


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