The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 14, 2022

Filed:

Jun. 16, 2017
Applicant:

Microsoft Technology Licensing, Llc, Redmond, WA (US);

Inventor:

Kris K. Ganjam, Seattle, WA (US);

Assignee:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6869 (2018.01); G16B 25/00 (2019.01); G06N 3/12 (2006.01); C12Q 1/6897 (2018.01); C12N 15/90 (2006.01); G06F 21/62 (2013.01); G06F 21/60 (2013.01); G16B 25/10 (2019.01); C12Q 1/6806 (2018.01); G06F 9/448 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6869 (2013.01); C12N 15/902 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6897 (2013.01); G06F 9/4498 (2018.02); G06F 21/602 (2013.01); G06F 21/6218 (2013.01); G06N 3/123 (2013.01); G16B 25/00 (2019.02); G16B 25/10 (2019.02); C12Q 2563/179 (2013.01);
Abstract

Gene expression can be identified by analyzing a DNA sequence. The DNA sequence can include a barcode sequence that corresponds to a particular gene. The barcode sequence can be produced during the expression of a gene by first adding a Homologous Directed Repair (HDR) template including the barcode sequence into the DNA sequence of the gene and then splicing the barcode sequence out of an RNA precursor during the expression of the gene. As the barcode sequence is made available from the RNA precursor, it can be added to the DNA strand using HDR. The resulting DNA strand can be sequenced and the sequence data can be analyzed to identify the barcode sequence within the DNA sequence, which provides an indicator of the expression of the gene in DNA rather than RNA.


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