The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 14, 2022
Filed:
Apr. 29, 2019
Applicant:
President and Fellows of Harvard College, Cambridge, MA (US);
Inventors:
George M. Church, Brookline, MA (US);
Prashant G. Mali, La Jolla, CA (US);
Luhan Yang, Somerville, MA (US);
Assignee:
President and Fellows of Harvard College, Cambridge, MA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/00 (2006.01); C12N 15/85 (2006.01); C12N 15/10 (2006.01); C12N 15/63 (2006.01); C12N 15/90 (2006.01); C12N 15/01 (2006.01); C12N 15/81 (2006.01); C12N 15/82 (2006.01); C12N 15/87 (2006.01); C12N 9/22 (2006.01);
U.S. Cl.
CPC ...
C12N 15/85 (2013.01); C12N 9/22 (2013.01); C12N 15/01 (2013.01); C12N 15/10 (2013.01); C12N 15/102 (2013.01); C12N 15/1024 (2013.01); C12N 15/63 (2013.01); C12N 15/81 (2013.01); C12N 15/8201 (2013.01); C12N 15/87 (2013.01); C12N 15/90 (2013.01); C12N 15/907 (2013.01); C12N 2310/20 (2017.05); C12N 2800/80 (2013.01); C12N 2810/55 (2013.01); C12Y 301/00 (2013.01);
Abstract
A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.