The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 07, 2022

Filed:

Dec. 15, 2016
Applicant:

Sangamo Biosciences, Inc., Richmond, CA (US);

Inventors:

Gary K. Lee, Richmond, CA (US);

David Paschon, Richmond, CA (US);

Lei Zhang, Richmond, CA (US);

Assignee:

Sangamo Therapeutics, Inc., Brisbane, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/62 (2006.01); C12N 15/63 (2006.01); A61K 48/00 (2006.01); C12N 15/52 (2006.01); C12N 5/073 (2010.01); C12N 5/0735 (2010.01); C12N 5/0783 (2010.01); C12N 5/074 (2010.01); C12N 9/16 (2006.01);
U.S. Cl.
CPC ...
C12N 15/52 (2013.01); C12N 5/0603 (2013.01); C12N 5/0606 (2013.01); C12N 5/0636 (2013.01); C12N 5/0696 (2013.01); C12N 9/16 (2013.01); C12N 15/62 (2013.01);
Abstract

Disclosed herein are methods and compositions for inactivating TCR genes, using engineered nucleases comprising at least one DNA binding domain and a cleavage domain or cleavage half-domain in conditions able to preserve cell viability. Polynucleotides encoding nucleases, vectors comprising polynucleotides encoding nucleases and cells comprising polynucleotides encoding nucleases and/or cells comprising nucleases are also provided. Disclosed herein are also methods and compositions for expressing a functional exogenous TCR in the absence of endogenous TCR expression in T lymphocytes, including lymphocytes with a central memory phenotype. Polynucleotides encoding exogenous TCR, vectors comprising polynucleotides encoding exogenous TCR and cells comprising polynucleotides encoding exogenous TCR and/or cells comprising exogenous TCR are also provided.


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