The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 07, 2022

Filed:

Sep. 29, 2017
Applicant:

Wisconsin Alumni Research Foundation, Madison, WI (US);

Inventors:

Mitch James Biermann, Madison, WI (US);

Timothy Joseph Kamp, Madison, WI (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); C12N 5/077 (2010.01); A61K 35/34 (2015.01); C12N 5/0735 (2010.01); C12N 5/074 (2010.01);
U.S. Cl.
CPC ...
C12N 5/0657 (2013.01); A61K 35/34 (2013.01); C12N 5/0606 (2013.01); C12N 5/0696 (2013.01); C12N 2501/056 (2013.01); C12N 2501/33 (2013.01); C12N 2501/415 (2013.01); C12N 2501/50 (2013.01); C12N 2501/727 (2013.01); C12N 2506/02 (2013.01); C12N 2506/45 (2013.01);
Abstract

An improvement to the GiWi protocol for differentiating human pluripotent cells to developmentally mature cardiomyocytes includes a step of activating innate immunity in mesoderm stage cells in the in vitro differentiation culture. When the mesoderm cells, which are precursors to cardiac progenitor cells, are primed by exposure to an activator of innate immunity, a population of cardiomyocytes is generated that is more developmentally mature than is generated in the GiWi protocol without the primed step. Also provided herein are in vitro ventricular conductive microtissues and isolated, in vitro populations of ventricular conduction system-like cells and methods for making the same.


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