The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 31, 2022
Filed:
Mar. 09, 2020
Roche Sequencing Solutions, Inc., Pleasanton, CA (US);
Kapa Biosystems, Inc., Wilmington, MA (US);
Daleen Badenhorst, Wellington, CA;
Richard Dannebaum, Pleasant Hill, CA (US);
Ashley Hayes, San Francisco, CA (US);
Monica Herrera, Livermore, CA (US);
Severine Margeridon, Castro Valley, CA (US);
Martin Ranik, Cape Town, ZA;
ROCHE SEQUENCING SOLUTIONS, INC., Pleasanton, CA (US);
KAPA BIOSYSTEMS, INC., Wilmington, MA (US);
Abstract
The invention is a novel method of making and using a library such as a sequencing library of single stranded circular nucleic acid templates via splint ligation. In particular, disclosed are methods of making circular target nucleic acid molecules and libraries of such molecules for downstream analysis such as nucleic acid sequencing. The method comprises the steps of adding universal sequences to nucleic acid molecules, rendering single-stranded these nucleic acid molecules with universal sequences on their ends by contacting with a probe complementary to at least a portion of the universal sequences, and allowing the hybridized probe to enable circularization and formation of single-stranded circular (sscDNA) molecules.