The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 24, 2022
Filed:
Nov. 08, 2018
Applicants:
Blue Sky Vaccines Gmbh, Vienna, AT;
Bia Separations D.o.o, Ljubljana, SI;
Inventors:
Markus Wolschek, Vienna, AT;
Manfred Reiter, Vienna, AT;
Ales Strancar, Ajdovscina, SI;
Mojca Tajnik Sbaizero, Bagnaria Arsa, IT;
Assignee:
BLUESKY IMMUNOTHERAPIES GMBH, Vienna, AT;
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C07K 14/00 (2006.01); A61K 38/00 (2006.01); C07H 21/04 (2006.01); C07K 14/765 (2006.01); C12Q 1/68 (2018.01); C12N 7/00 (2006.01);
U.S. Cl.
CPC ...
C12N 7/00 (2013.01); C12N 2760/16152 (2013.01); C12N 2760/16252 (2013.01);
Abstract
The present invention refers to a process for purifying virus particles from cell culture, comprising the steps of subjecting the cell culture to centrifugation to get a supernatant fraction of virus particles, incubating said fraction with a nuclease, diluting the fraction with low conductivity buffer, subjecting said diluted fraction to a SO3 chromatography step, performing a washing step with low conductivity buffer, and eluting virus particles.