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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 11339371 B1

PDFFull Text
Date of Patent:
May. 24, 2022

Filed:

Jul. 22, 2013

Method for inducing pluripotent stem cells to differentiate into ventricular myocytes in vitro

Applicant:

Institute of Biophysics, Chinese Academy of Sciences, Beijing, CN;

Inventor:

Yue Ma, Beijing, CN;

Assignee:

INSTITUTE OF BIOPHYSICS, CHINESE ACADEMY OF SCIENCES, Beijing, CN;

Attorney:

Rimon, P.C.

Primary Examiner:

Valarie E Bertoglio

Int. Cl.
CPC ...
C12N 5/077 (2010.01); A61K 35/34 (2015.01); A61K 35/28 (2015.01); A61K 35/545 (2015.01);
U.S. Cl.
CPC ...
C12N 5/0657 (2013.01); A61K 35/28 (2013.01); A61K 35/34 (2013.01); A61K 35/545 (2013.01); C12N 2501/115 (2013.01); C12N 2501/155 (2013.01); C12N 2501/16 (2013.01); C12N 2501/385 (2013.01); C12N 2501/40 (2013.01); C12N 2501/415 (2013.01); C12N 2503/02 (2013.01); C12N 2506/02 (2013.01); C12N 2506/45 (2013.01);
Abstract

Provided in the present invention is a method for inducing pluripotent stem cells to differentiate into ventricular myocytes in vitro, which is achieved by maintaining, amplifying and culturing pluripotent stem cells in vitro, adding a substance capable of activating the Smad1/5/8 signaling pathway directly or indirectly into the culture medium when pluripotent stem cells are in the middle stage of myocardial differentiation, i.e. the period of differentiating into cardiac muscle cells from mesoderm cells or myocardial precursor cells, which enables stem cells to differentiate into ventricular myocytes directionally. Ventricular myocytes with biological activity and function are obtained successfully by means of the method of the present invention, which reveals the regulatory mechanism during differentiation of myocardial precursor cells into ventricular myocytes; moreover, the human ventricular myocytes obtained via differentiation can be widely used in treating myocardial infarction by cell transplantation, in toxicological analysis of the heart and in the development of heart-related drugs.

Find Patent Forward Citations

Ma, Yue. (2022). Method for inducing pluripotent stem cells to differentiate into ventricular myocytes in vitro (U.S. Patent No. 11339371). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/11339371

Data source: USPTO (United States Patent and Trademark Office) public patent records. Weekly synchronization. How IDiyas builds this profile

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