The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 17, 2022

Filed:

Apr. 09, 2019
Applicant:

Dh Technologies Development Pte. Ltd., Singapore, SG;

Inventors:

Takashi Baba, Richmond Hill, CA;

Pavel Ryumin, Toronto, CA;

William M. Loyd, Sugar Land, TX (US);

Assignee:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
H01J 49/40 (2006.01); G16B 40/10 (2019.01); G01N 33/68 (2006.01); G01N 33/577 (2006.01); H01J 49/00 (2006.01); G16B 30/10 (2019.01);
U.S. Cl.
CPC ...
H01J 49/40 (2013.01); G01N 33/577 (2013.01); G01N 33/6848 (2013.01); G16B 30/10 (2019.02); G16B 40/10 (2019.02); H01J 49/0045 (2013.01);
Abstract

A separation device separates an unknown intact mAb or reduced mAb subunits of a known mAb class from a sample. An ion source device ionizes the mAb. A mass spectrometer fragments the ionized mAb using an ECD device and mass analyzes resulting product ions using a mass analyzer, producing one or more product ion spectra. Theoretical product ion peaks are calculated for one or more constant portions of the mAb class. The theoretical product ion peaks are removed from the one or more product ion spectra, producing one or more difference product ion spectra. De novo sequencing is applied to the one or more difference product ion spectra, producing one or more candidate sequences for one or more variable portions of the mAb. A genome database is searched for matches to the one or more candidate sequences, producing one or more matched sequences for the one or more variable portions.


Find Patent Forward Citations

Loading…