The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 17, 2022
Filed:
Jun. 25, 2020
Biofidelity Ltd, Cambridge, GB;
Barnaby Balmforth, Cambridge, GB;
Magdalena Stolarek-Januszkiewicz, Cambridge, GB;
Ana Silva-Weatherley, Cambridge, GB;
Paulina Powalowska, Cambridge, GB;
BIOFIDELITY LTD, Cambridge, GB;
Abstract
Methods of detecting target polynucleotide sequences may include introducing one or more nucleic acid analytes to a first reaction mixture comprising a single-stranded probe oligonucleotide A, a pyrophosphorolysing enzyme, and a ligase. The analyte may anneal to the single-stranded probe oligonucleotide Ato create a first intermediate product which is at least partially double-stranded, where the 3' end of Aforms a double-stranded complex with the analyte and where Ais pyrophosphorylsed in the 3′-5′ direction from the 3′ end to create at least a partially digested strand A. Amay undergo ligation to form oligonucleotide A. The methods may also include detecting a signal derived from the formed oligonucleotides, and inferring therefrom the presence or absence of the target polynucleotide sequence in the analyte.