The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 17, 2022
Filed:
Sep. 28, 2018
Enzymaster (Ningbo) Bio-engineering Co., Ltd., Ningbo, CN;
Haibin Chen, Ningbo, CN;
Yong Koy Bong, Ningbo, CN;
Wenhui Zheng, Ningbo, CN;
Zhaoqi Zhang, Ningbo, CN;
Kuifang He, Ningbo, CN;
Chengxiao Zhang, Ningbo, CN;
Lei Sun, Ningbo, CN;
Yingxin Zhang, Ningbo, CN;
Enzymaster (Ningbo) Bio-Engineering Co., Ltd., Ningbo, CN;
Abstract
The present invention provides amino acid sequences of engineered decarboxylase polypeptides that are useful for catalyzing the decarboxylation of L-aspartate to produce β-alanine, and the preparation process of engineered decarboxylase polypeptides as well as reaction process under industrial-relevant conditions. The present disclosure also provides polynucleotide sequences encoding engineered decarboxylase polypeptides, engineered host cells capable of expressing engineered decarboxylase polypeptides, and methods of producing β-alanine using the engineered cells. Compared to the wild-type decarboxylase, the engineered decarboxylase polypeptide provided by the invention has better activity and stability, and overcomes the inhibition by L-aspartic acid and/or β-alanine. The use of the engineered polypeptides of the present invention for the preparation of β-alanine results in higher unit activity, lower cost, and has good industrial application prospects.