The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 17, 2022

Filed:

Jul. 02, 2015
Applicant:

Center for Excellence IN Molecular Cell Science, Chinese Academy of Sciences, Shanghai, CN;

Inventors:

Jinsong Li, Shanghai, CN;

Yuxuan Wu, Shanghai, CN;

Cuiqing Zhong, Shanghai, CN;

Qi Yin, Shanghai, CN;

Zhenfei Xie, Shanghai, CN;

Meizhu Bai, Shanghai, CN;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
A01K 67/00 (2006.01); A01K 67/027 (2006.01); C12N 15/85 (2006.01); C12N 15/113 (2010.01); C12N 15/877 (2010.01); C12N 5/0735 (2010.01); C12N 15/873 (2010.01);
U.S. Cl.
CPC ...
A01K 67/0276 (2013.01); C12N 5/0606 (2013.01); C12N 15/113 (2013.01); C12N 15/85 (2013.01); C12N 15/873 (2013.01); C12N 15/8775 (2013.01); A01K 2217/075 (2013.01); A01K 2217/15 (2013.01); A01K 2227/105 (2013.01); C12N 2500/10 (2013.01); C12N 2500/50 (2013.01); C12N 2501/065 (2013.01); C12N 2501/235 (2013.01); C12N 2501/727 (2013.01); C12N 2502/99 (2013.01); C12N 2740/16043 (2013.01);
Abstract

The present invention relates to an AG-haESCs in which H19 DMR and IG-DMR are knocked out, a method for preparing the AG-haESCs, and use of the AG-haESCs in constructing a genetically modified semi-cloned animal and a library of a genetically modified semi-cloned animal. The AG-haESCs is capable of obtaining characteristics resembling a round spermatid, and upon injection into an oocyte, a viable SC mouse is stably obtained. The present invention is capable of being effectively used in multi-gene genetic manipulation, advancing the acquisition of animals with multiple genetic modifications.


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