The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 10, 2022

Filed:

Dec. 02, 2020
Applicant:

Exuma Biotech Corp., West Palm Beach, FL (US);

Inventors:

Gregory Ian Frost, West Palm Beach, FL (US);

James Joseph Onuffer, Jr., Alameda, CA (US);

Ghiabe H. Guibinga, San Diego, CA (US);

Farzad Haerizadeh, San Diego, CA (US);

Frederic Vigant, Villers-sous-Saint-Leu, FR;

Anirban Kundu, West Bay, KY;

Assignee:

Exuma Biotech Corp., West Palm Beach, FL (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/867 (2006.01); C07K 14/005 (2006.01); C12N 15/86 (2006.01); C07K 14/705 (2006.01); C07K 16/28 (2006.01); C07K 14/725 (2006.01); C07K 14/54 (2006.01); A61K 47/69 (2017.01); A61K 35/17 (2015.01); C12N 7/00 (2006.01); A61K 38/00 (2006.01); A61K 39/00 (2006.01);
U.S. Cl.
CPC ...
C07K 14/005 (2013.01); A61K 35/17 (2013.01); A61K 47/6901 (2017.08); C07K 14/5418 (2013.01); C07K 14/7051 (2013.01); C07K 14/70517 (2013.01); C07K 14/70578 (2013.01); C07K 14/70596 (2013.01); C07K 16/2803 (2013.01); C07K 16/2809 (2013.01); C12N 7/00 (2013.01); C12N 15/86 (2013.01); A61K 38/00 (2013.01); A61K 2039/505 (2013.01); C07K 2317/622 (2013.01); C07K 2317/76 (2013.01); C07K 2319/02 (2013.01); C07K 2319/03 (2013.01); C07K 2319/30 (2013.01); C07K 2319/33 (2013.01); C12N 2740/10022 (2013.01); C12N 2740/10043 (2013.01);
Abstract

The present disclosure provides methods and compositions for genetically modifying lymphocytes, for example T cells and/or NK cells, in shorter times than previously and/or in whole blood or a component thereof. In some embodiments a lymphodepletion filter assembly is used before or after forming a reaction mixture where lymphocytes are contacted with recombinant retroviral particles in a closed system, to genetically modify the lymphocytes.


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