The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 03, 2022
Filed:
Mar. 12, 2020
Michael Edward Hogan, Stony Brook, NJ (US);
Benjamin Alan Katchman, Tucson, AZ (US);
Candy Mavis Rivas, Tucson, AZ (US);
Yasmine Eve Baca, San Tan Valley, AZ (US);
Michael Edward Hogan, Stony Brook, NJ (US);
Benjamin Alan Katchman, Tucson, AZ (US);
Candy Mavis Rivas, Tucson, AZ (US);
Yasmine Eve Baca, San Tan Valley, AZ (US);
PathogenDx, Inc., Scottsdale, AZ (US);
Abstract
Provided herein are methods for isolating nucleic acids from intact cells in a sample of intact cells, contamination dead cells, cell debris, and biofilm using two separation steps, either by centrifugation or filtration, performed in sequentially. Also provided is a method for isolating nucleic acids from intact cells using a first separation step followed by treatment with a nuclease and then a second separating step. Provided herein is a related method for isolating DNA from intact cells using a nuclease that produces DNA cuts on double stranded DNA, followed by a second separating step.