The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 26, 2022
Filed:
Dec. 05, 2018
Feng Chi Biotech Corporation, Taipei, TW;
FENG CHI BIOTECH CORPORATION, Taipei, TW;
Abstract
A method for detecting a SNP site on a SMA gene is disclosed, and includes steps of: (S) performing a PCR for amplifying a nucleic a nucleic acid fragment containing a SNP site; (S) performing a dephosphorylation reaction on the nucleic acid fragment; (S) performing an extension reaction on the nucleic acid fragment, wherein the SNP site is identified by using an extension primer, a 3'-end of the extension primer is extended by a single nucleotide which is complementary to a base of the SNP site, and thus an extended extension primer is obtained; (S) performing a purification reaction; and (S) measuring a molecular weight of the extended extension primer, and determining a type of a base of the single nucleotide based upon the molecular weight, thereby determining whether deletion occurs to the SNP site.