The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 26, 2022

Filed:

Aug. 31, 2016
Applicant:

University of Louisville Research Foundation, Inc., Louisville, KY (US);

Inventors:

Mariusz Z. Ratajczak, Louisville, KY (US);

Janina Ratajczak, Louisville, KY (US);

Magdalena Kucia, Louisville, KY (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 31/00 (2006.01); G01N 33/53 (2006.01); C12N 5/0775 (2010.01); G01N 33/569 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0663 (2013.01); C12N 5/0665 (2013.01); G01N 33/56966 (2013.01); C12N 2501/31 (2013.01); C12N 2501/315 (2013.01); C12N 2501/392 (2013.01);
Abstract

Provided are methods for isolating subpopulations of stem cells. In some embodiments, the presently disclosed methods include selecting subsets of cells that are positive for CD34 or Sca-1, are further positive for one or more of FSHR, LHCGR, PRLR, AR, ESRα, ESRβ, and PGR; and are negative for each of CD45R/B220, Gr-1, TCRαβ, TCRγδ, CD11b, and Ter-119. In some embodiments, the subpopulations are further fractioning into CD45and CD45fractions. Also provided are populations of stem cells isolated by the presently disclosed methods, compositions that include the presently disclosed subpopulations in pharmaceutically acceptable carriers, methods for expanding stem cells, methods for stimulating proliferation of MSCs, methods for treating subjects suffering from exposure to radiation, and methods for producing gametes in vitro.


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