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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 11312745 B1

PDFFull Text
Date of Patent:
Apr. 26, 2022

Filed:

Jul. 21, 2017

Methods of purifying fc-containing proteins

Applicant:

Amgen Inc., Thousand Oaks, CA (US);

Inventor:

John K. Kawooya, Moorpark, CA (US);

Assignee:

AMGEN INC., Thousand Oaks, CA (US);

Attorney:

Susan E. Lingenfelter

Primary Examiner:

Daniel E Kolker

Assistant Examiner:

James L Rogers

Int. Cl.
CPC ...
C07K 1/22 (2006.01); B01D 15/34 (2006.01); B01D 15/38 (2006.01); B01D 15/42 (2006.01); C07K 16/00 (2006.01); C07K 1/34 (2006.01);
U.S. Cl.
CPC ...
C07K 1/22 (2013.01); B01D 15/34 (2013.01); B01D 15/3809 (2013.01); B01D 15/426 (2013.01); C07K 1/34 (2013.01); C07K 16/00 (2013.01); C07K 2317/31 (2013.01); C07K 2317/35 (2013.01); C07K 2317/60 (2013.01); C07K 2317/622 (2013.01);
Abstract

The present invention relates to methods of purifying proteins containing Fc regions, such as antibodies and Fc fusion proteins. In particular, the present invention relates to a purification method resulting in reduced levels of aggregate protein comprising adsorbing an Fc region-containing protein to a temperature-responsive protein A resin and eluting the protein from the resin at a temperature below 35 C with an elution buffer comprising a chaotropic agent, a sugar alcohol, and at least one amino acid. Methods of separating fully assembled antibodies from half antibody forms thereof using the elution buffer are also described.

Find Patent Forward Citations

Kawooya, John. (2022). Methods of purifying fc-containing proteins (U.S. Patent No. 11312745). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/11312745

Data source: USPTO (United States Patent and Trademark Office) public patent records. Weekly synchronization. How IDiyas builds this profile

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