The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 26, 2022

Filed:

May. 13, 2014
Applicant:

Cellectis, Paris, FR;

Inventors:

Roman Galetto, Paris, FR;

Agnes Gouble, Paris, FR;

Stephanie Grosse, Saint Cyr sur Morin, FR;

Cécile Schiffer-Mannioui, Villiers sur Marne, FR;

Laurent Poirot, Paris, FR;

Andrew Scharenberg, Seattle, WA (US);

Julianne Smith, Le Plessis Robinson, FR;

Assignee:

CELLECTIS, Paris, FR;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A61K 48/00 (2006.01); C07H 21/04 (2006.01); A61K 35/17 (2015.01); C12N 9/22 (2006.01); C12N 5/0783 (2010.01); A01K 67/00 (2006.01); A61K 35/12 (2015.01);
U.S. Cl.
CPC ...
A61K 35/17 (2013.01); C12N 5/0636 (2013.01); C12N 9/22 (2013.01); A61K 2035/124 (2013.01); C07K 2319/80 (2013.01); C12N 2501/39 (2013.01); C12N 2501/51 (2013.01); C12N 2501/515 (2013.01); C12N 2501/599 (2013.01); C12N 2502/99 (2013.01); C12N 2510/00 (2013.01);
Abstract

The present invention relates to methods for developing engineered T-cells for immunotherapy and more specifically to methods for modifying T-cells by inactivating at immune checkpoint genes, preferably at least two selected from different pathways, to increase T-cell immune activity. This method involves the use of specific rare cutting endonucleases, in particular TALE-nucleases (TAL effector endonuclease) and polynucleotides encoding such polypeptides, to precisely target a selection of key genes in T-cells, which are available from donors or from culture of primary cells. The invention opens the way to highly efficient adoptive immunotherapy strategies for treating cancer and viral infections.


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