The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 26, 2022

Filed:

Oct. 01, 2015
Applicant:

National Agriculture and Food Research Organization, Tsukuba, JP;

Inventors:

Sachiko Machida, Tsukuba, JP;

Miyuki Kuramochi, Tsukuba, JP;

Mayumi Kameyama, Tsukuba, JP;

Mari Yamamoto, Tsukuba, JP;

Toshiro Kobori, Tsukuba, JP;

Hideki Sezutsu, Tsukuba, JP;

Ken-ichiro Tatematsu, Tsukuba, JP;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
A01K 67/027 (2006.01); C07K 16/18 (2006.01); C07K 14/705 (2006.01); A01K 67/033 (2006.01); C07K 16/00 (2006.01); C07K 14/47 (2006.01); C12N 9/00 (2006.01); G01N 33/92 (2006.01);
U.S. Cl.
CPC ...
A01K 67/0275 (2013.01); A01K 67/0339 (2013.01); C07K 14/4726 (2013.01); C07K 14/705 (2013.01); C07K 14/70503 (2013.01); C07K 16/00 (2013.01); C07K 16/005 (2013.01); C07K 16/18 (2013.01); C12N 9/93 (2013.01); C12Y 603/04015 (2013.01); G01N 33/92 (2013.01); A01K 2227/706 (2013.01); A01K 2267/01 (2013.01); C07K 2317/21 (2013.01); C07K 2317/622 (2013.01); C07K 2319/20 (2013.01); G01N 2333/705 (2013.01); G01N 2440/00 (2013.01); G01N 2800/323 (2013.01);
Abstract

The present invention pertains to a silkworm-type biotinylated CTLD14 or sRAGE and a method for manufacturing the same. One embodiment of the present invention provides a method for manufacturing biotinylated proteins, wherein the method includes A) a step for inserting a nucleic acid molecule for coding biotin ligase and protein in a coexpressable manner into a silkworm or a living organism that imparts sugar chains that are the same as the sugar chains of the silkworm, B) a step for causing the biotin ligase and protein to be expressed by disposing the silkworm or the living organism that imparts sugar chains that are the same as the sugar chains of the silkworm to conditions with which the nucleic acid molecule will carry out expression, and C) a step for administering biotin to the living organism and obtaining the biotinylated protein.


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