The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 19, 2022

Filed:

May. 03, 2019
Applicant:

Akoya Biosciences, Inc., Menlo Park, CA (US);

Inventors:

Peter J. Miller, Hopkinton, MA (US);

Kent S. Johnson, Cambridge, MA (US);

Carla Coltharp, Waltham, MA (US);

Assignee:

Akoya Biosciences, Inc., Menlo Park, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 21/64 (2006.01); G06T 7/194 (2017.01); G06T 7/00 (2017.01); G01J 3/28 (2006.01);
U.S. Cl.
CPC ...
G01N 21/6458 (2013.01); G01J 3/28 (2013.01); G01J 3/2823 (2013.01); G01N 21/6428 (2013.01); G06T 7/0012 (2013.01); G06T 7/194 (2017.01); G01J 2003/2826 (2013.01); G01N 2021/6419 (2013.01); G01N 2021/6421 (2013.01); G06T 2207/10036 (2013.01); G06T 2207/10056 (2013.01); G06T 2207/10064 (2013.01); G06T 2207/30024 (2013.01);
Abstract

The disclosure features methods that include exposing a biological sample to illumination light and measuring light emission from the sample to obtain N sample images, where each sample image corresponds to a different combination of a wavelength band of the illumination light and one or more wavelength bands of the light emission, where the one or more wavelength bands of the light emission define a wavelength range, and where N>1, and exposing the sample to illumination light in a background excitation band and measuring light emission from the sample in a background spectral band to obtain a background image of the sample, where the background spectral band corresponds to a wavelength within the wavelength range.


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