The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 19, 2022
Filed:
Sep. 05, 2019
Applicant:
Pharmazell Gmbh, Raubling, DE;
Inventors:
Werner Hummel, Titz, DE;
Daniel Bakonyi, Cologne, DE;
Assignee:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/04 (2006.01); C12N 15/00 (2006.01); C12N 15/53 (2006.01); C12N 1/20 (2006.01); C12P 33/00 (2006.01); C12P 13/02 (2006.01); C12P 33/02 (2006.01);
U.S. Cl.
CPC ...
C12P 33/02 (2013.01); C12N 9/0006 (2013.01); C12Y 101/0105 (2013.01); C12Y 101/01159 (2013.01); C12Y 101/01225 (2013.01);
Abstract
The invention provides novel 3α-hydroxysteroid dehydrogenase mutants, sequences that code for these enzyme mutants, methods for producing the enzyme mutants, and the use thereof in enzymatic reactions of cholic acid compounds, and in particular in the production of ursodeoxycholic acid (UDCA). The invention further provides processes for the synthesis of UDCA using the enzyme mutants and the production of UDCA using recombinant microorganisms that have been subjected to multiple modifications.