The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 12, 2022

Filed:

Oct. 12, 2018
Applicant:

Lawrence Livermore National Security, Llc, Livermore, CA (US);

Inventors:

Matthew A. Coleman, Oakland, CA (US);

Paul D. Hoeprich, Pleasanton, CA (US);

Brent W. Segelke, San Ramon, CA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/58 (2006.01); C07K 14/705 (2006.01); C12P 21/02 (2006.01); G01N 33/543 (2006.01); C07K 17/02 (2006.01); C07K 14/775 (2006.01);
U.S. Cl.
CPC ...
G01N 33/587 (2013.01); C07K 14/705 (2013.01); C12P 21/02 (2013.01); G01N 33/54346 (2013.01); G01N 33/582 (2013.01); C07K 14/775 (2013.01); C07K 17/02 (2013.01);
Abstract

Provided herein are methods and systems for the production of a nanolipoprotein particle (NLP) that includes a scaffold protein a membrane forming lipid and optionally a target protein. At least one of the scaffold protein and target protein can be provided through an IVT system. The membrane forming lipid, scaffold protein and optionally the target protein can be assembled for a time and under conditions that allow obtaining high yield NLPs, NPLs with an increased solubility, an NLP of a controlled size, and/or an NLP having a size predetermined to include a pre-selected target protein.


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