The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 12, 2022
Filed:
Aug. 09, 2021
Applicant:
Zymergen Inc., Emeryville, CA (US);
Inventors:
Tara F. Mahoney, San Francisco, CA (US);
Amanda Renae Reider Apel, Alameda, CA (US);
Paloma Rueda Romero, Berkeley, CA (US);
Assignee:
Zymergen Inc., Emeryville, CA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/90 (2006.01); C12N 1/20 (2006.01); C12N 9/00 (2006.01);
U.S. Cl.
CPC ...
C12N 15/90 (2013.01); C12N 1/20 (2013.01); C12N 9/93 (2013.01); C12Y 601/0102 (2013.01); C12N 2501/999 (2013.01);
Abstract
The present disclosure is directed to methods of scarless genomic engineering in microorganisms, such as, and provides for new molecular tools and methods which enable scarless genetic editing using at least one counterselectable marker that has been codon optimized for the microorganism. The disclosure allows for the high-throughput introduction of stable genetic edits to a genome using either plasmid or linear DNA constructs for genetic engineering.