The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 05, 2022

Filed:

Dec. 13, 2019
Applicant:

Sensor Kinesis Corporation, Inglewood, CA (US);

Inventors:

Josh Shachar, Santa Monica, CA (US);

Roger Kornberg, Atherton, CA (US);

Assignee:

Autonomous Medical Devices Inc., Santa Ana, CA (US);

Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N 33/00 (2006.01); G01N 33/543 (2006.01); G01N 33/541 (2006.01); G01N 33/49 (2006.01); B01L 3/00 (2006.01);
U.S. Cl.
CPC ...
G01N 33/54333 (2013.01); B01L 3/502715 (2013.01); G01N 33/491 (2013.01); G01N 33/541 (2013.01); G01N 33/5434 (2013.01); B01L 2300/0636 (2013.01); B01L 2400/0436 (2013.01); B01L 2400/06 (2013.01); G01N 2446/20 (2013.01); G01N 2446/90 (2013.01); G01N 2458/10 (2013.01);
Abstract

The invention includes a method of assaying an analyte in a sample in a portable, handheld microfluidic reader. The method includes the steps of: inserting the sample in the reader; capturing the analyte with a first antibody having a DNA tag attached thereto; capturing the analyte in the sample with a second antibody attached to a surface or having a magnetic nanoparticle (MNP) attached thereto; where a sandwich including the magnetic nanoparticle, first and second antibodies, the analyte and the DNA tag is formed; replicating the DNA tag using isothermal amplification to a predetermined amount of DNA tags detectable by a detector sufficient to overcome the minimal mass sensitivity limitations of the detector; and measuring the amount of replicated DNA tags using the detector. The invention also includes an apparatus or handheld portable field microfluidic reader in which the method is performed.


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