The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 05, 2022

Filed:

Nov. 07, 2018
Applicant:

Life Technologies Corporation, Carlsbad, CA (US);

Inventors:

Abraham Rosenbaum, Waterbury, CT (US);

Collyn Seeger, Middletown, CT (US);

Jeremy Gray, Larkspur, CA (US);

Hua Yu, Guilford, CT (US);

Assignee:

Life Technologies Corporation, Carlsbad, CA (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/686 (2018.01); C12Q 1/6806 (2018.01); C12Q 1/6834 (2018.01); C12Q 1/6853 (2018.01); C12Q 1/6876 (2018.01); C12Q 1/6874 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/686 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6834 (2013.01); C12Q 1/6853 (2013.01); C12Q 1/6874 (2013.01); C12Q 1/6876 (2013.01); C12Q 2600/16 (2013.01);
Abstract

The present disclosure provides methods, compositions and kits as well as systems for manipulating nucleic acids, including implementing isothermal amplification, such as recombinase-polymerase amplification (RPA), of a nucleic acid template using a pre-seeded solid support. Provided are rapid and efficient methods for generating template nucleic acid molecules comprising specific nucleotide sequence bound to solid support. Such methods can be used, for example, in manipulating nucleic acids in preparation for analysis methods that utilize monoclonal populations of nucleic acids.


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