The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 05, 2022
Filed:
Sep. 10, 2019
Applicant:
Osaka University, Osaka, JP;
Inventors:
Assignee:
OSAKA UNIVERSITY, Osaka, JP;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/02 (2006.01); C12N 15/09 (2006.01); A61K 38/00 (2006.01); C12N 5/00 (2006.01); C07K 14/47 (2006.01); C07K 14/76 (2006.01); C07K 19/00 (2006.01); C12P 21/02 (2006.01); C07K 14/00 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0018 (2013.01); A61K 38/00 (2013.01); C07K 14/4702 (2013.01); C07K 14/76 (2013.01); C07K 19/00 (2013.01); C12N 15/09 (2013.01); C12P 21/02 (2013.01); C07K 14/00 (2013.01); C12N 2501/415 (2013.01);
Abstract
A method for producing a Wnt protein-afamin complex, the method comprising the steps of culturing Wnt protein-expressing cells in a culture medium containing a purified afamin or a recombinant afamin or co-culturing Wnt protein-expressing cells and recombinant afamin-expressing cells or culturing cells expressing both a Wnt protein and afamin; obtaining the culture supernatant; and optionally performing affinity purification to obtain the Wnt protein-afamin complex from the culture supernatant.