The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 29, 2022

Filed:

Dec. 18, 2018
Applicants:

Cic Nanogune—asociación Centro DE Investigación Cooperativa En Nanociencias, Guipuzcoa, ES;

Universidad Del País Vasco, Vizcaya, ES;

Inventors:

Raúl Pérez-Jiménez, San Sebastian, ES;

Nerea Barruetabeña Garate, Guipuzcoa, ES;

Mᵃ Aranzazu Eceiza Mendiguren, Donostia-San Sebastián, ES;

Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P 7/08 (2006.01); C12P 19/14 (2006.01); C12N 9/42 (2006.01); C12P 7/10 (2006.01); C12N 9/24 (2006.01);
U.S. Cl.
CPC ...
C12P 7/08 (2013.01); C12N 9/2437 (2013.01); C12N 9/2445 (2013.01); C12P 7/10 (2013.01); C12P 19/14 (2013.01); C12Y 110/03002 (2013.01); C12Y 302/01004 (2013.01); C12Y 302/01008 (2013.01); C12Y 302/01021 (2013.01); C12Y 302/01091 (2013.01);
Abstract

The invention relates to a polypeptide comprising an exoglucanase catalytic domain comprising a sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2 and SEQ ID NO: 3, and to a polypeptide having beta-glucosidase activity comprising a sequence selected from the group consisting of SEQ ID NO: 6, SEQ ID NO: 7 and SEQ ID NO: 8, and to functionally equivalent variants thereof that maintain or improve their catalytic activity. Additionally, the invention relates to an enzyme cocktail comprising said polypeptide(s) and an endoglucanase. Further, the invention also relates to methods for hydrolysing cellulose to cellobiose and/or cellotetraose, cellobiose and/or cellotetraose to glucose and cellulose to glucose, and to produce bioethanol, using the polypeptides or enzyme cocktails of the invention, and to the uses of the polypeptides and enzyme cocktails of the invention for hydrolysing cellulose to cellobiose and/or cellotetraose, cellobiose and/or cellotetraose to glucose and cellulose to glucose, and to produce bioethanol.


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