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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 11286504 B1

PDFFull Text
Date of Patent:
Mar. 29, 2022

Filed:

Mar. 08, 2019

Method to produce protein in's sleeping spores by transformation of ssrna

Applicant:

Fuzhou University, Fuzhou, CN;

Inventor:

Jun Lin, Fuzhou, CN;

Assignee:

FUZHOU UNIVERSITY, Fuzhou, CN;

Attorney:

W&KIP

Primary Examiner:

Paul J Holland

Int. Cl.
CPC ...
C12N 15/87 (2006.01); C12N 15/80 (2006.01); C12N 5/10 (2006.01); C12N 15/82 (2006.01); C12N 13/00 (2006.01); C12N 1/14 (2006.01); C12N 3/00 (2006.01); C07K 1/13 (2006.01); C12R 1/80 (2006.01);
U.S. Cl.
CPC ...
C12N 15/87 (2013.01); C12N 5/10 (2013.01); C12N 15/80 (2013.01); C12N 15/8206 (2013.01); C07K 1/13 (2013.01); C07K 2319/00 (2013.01); C07K 2319/60 (2013.01); C12N 1/14 (2013.01); C12N 1/145 (2021.05); C12N 3/00 (2013.01); C12N 13/00 (2013.01); C12R 2001/80 (2021.05);
Abstract

The present invention discloses A METHOD TO PRODUCE PROTEIN IN'S SLEEPING SPORES BY TRANSFORMATION OF SSRNA. The method includes three steps of culture ofand collection of spores, pretreatment ofspores, and electroporation ofspores by using HDEN method. In the present invention, non-germinated spores are used as a starting material for introduction of exogenous molecules. The exogenous protein coding single stranded RNA is introduced into the resting spores ofby employing the HDEN electrotransformation technique to express protein. The method of this invention is simple and fast, the effect is excellent, and the transformation rate reaches more than 90%.

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