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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 11286477 B1

PDFFull Text
Date of Patent:
Mar. 29, 2022

Filed:

May. 01, 2015

Preparation of libraries of protein variants expressed in eukaryotic cells and use for selecting binding molecules

Applicant:

Iontas Limited, Sawston, GB;

Inventors:

John McCafferty, Babraham, GB;

Michael Dyson, Cambridge, GB;

Kothai Parthiban, Cambridge, GB;

Assignee:

Iontas Limited, Sawston, GB;

Attorney:

Clark & Elbing LLP

Primary Examiner:

Quang Nguyen

Int. Cl.
CPC ...
C12Q 1/68 (2018.01); G01N 33/567 (2006.01); C12N 15/63 (2006.01); C12N 15/87 (2006.01); C12N 15/10 (2006.01); C12N 15/90 (2006.01); C07K 16/00 (2006.01); A61K 48/00 (2006.01);
U.S. Cl.
CPC ...
C12N 15/102 (2013.01); C07K 16/005 (2013.01); C12N 15/1037 (2013.01); C12N 15/907 (2013.01); C07K 2317/24 (2013.01); C07K 2317/622 (2013.01); C07K 2317/64 (2013.01); C07K 2319/03 (2013.01); C07K 2319/035 (2013.01); C07K 2319/036 (2013.01); C12N 2310/20 (2017.05); C12N 2800/30 (2013.01); C12N 2800/80 (2013.01);
Abstract

The invention relates to methods of producing eukaryotic cell libraries encoding a repertoire of binding molecules ('binders'), wherein the methods use a site-specific nuclease for targeted cleavage of cellular DNA to enhance site-specific integration of binder genes through endogenous cellular repair mechanisms. Populations of eukaryotic cells are produced in which a repertoire of genes encoding binders are integrated into a desired locus in cellular DNA (e.g., a genomic locus) allowing expression of the encoded binding molecule, thereby creating a population of cells expressing different binders.

Find Patent Forward Citations

McCafferty, John. (2022). Preparation of libraries of protein variants expressed in eukaryotic cells and use for selecting binding molecules (U.S. Patent No. 11286477). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/11286477

Data source: USPTO (United States Patent and Trademark Office) public patent records. Weekly synchronization. How IDiyas builds this profile

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