The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 29, 2022

Filed:

Dec. 20, 2013
Applicant:

Nederlandse Organisatie Voor Toegepast-natuurwetenschappelijk Onderzoek Tno, 's-Gravenhage, NL;

Inventors:

Govardus Adrianus Hubertus de Jong, 's-Gravenhage, NL;

Peter Geerdink, 's-Gravenhage, NL;

Paulus Josephus Theodorus Bussmann, 's-Gravenhage, NL;

Nienke Hylkema, 's-Gravenhage, NL;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A23J 1/00 (2006.01); C12N 9/88 (2006.01); A23J 3/22 (2006.01); A23J 3/14 (2006.01); B01D 11/00 (2006.01); B01D 61/14 (2006.01); B01D 15/08 (2006.01); A23P 30/40 (2016.01); A23L 33/105 (2016.01); A23L 33/185 (2016.01); A23L 29/10 (2016.01);
U.S. Cl.
CPC ...
A23J 1/006 (2013.01); A23J 1/007 (2013.01); A23J 3/14 (2013.01); A23J 3/22 (2013.01); A23L 29/10 (2016.08); A23L 33/105 (2016.08); A23L 33/185 (2016.08); A23P 30/40 (2016.08); B01D 11/00 (2013.01); B01D 15/08 (2013.01); B01D 61/145 (2013.01); B01D 61/147 (2013.01); C12N 9/88 (2013.01); A23V 2002/00 (2013.01); C12Y 401/01039 (2013.01);
Abstract

The invention relates to a method for obtaining a protein from a plant material, wherein the method comprises the steps of i) mechanically disrupting the plant cells to obtain a plant juice in the presence of a reducing agent, ii) treating the plant juice to cause aggregation of chloroplast membranes, iii) removing the aggregated chloroplast membranes by precipitation and/or microfiltration, iv) subjecting the plant juice to ultrafiltration, and v) subjecting the soluble plant protein concentrate to hydrophobic column adsorption to remove residual chlorophyll, phenolic compounds and off-odors in a single column passage. The present invention also pertains to an apparatus and system for plant protein isolation based on this method. The isolated proteins can be economically obtained in batch scale and in large scale. Further, the invention is directed to a protein obtained by the method of the invention, a food product comprising thereof, and a use thereof.


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