The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 22, 2022
Filed:
Sep. 06, 2018
On-chip Biotechnologies Co., Ltd., Tokyo, JP;
Shizuoka Prefecture, Shizuoka, JP;
Kazuo Takeda, Tokyo, JP;
Namiko Yamashita, Tokyo, JP;
Yuu Fujimura, Tokyo, JP;
Kaori Nishio, Tokyo, JP;
Yasuhiro Koh, Shizuoka, JP;
Masaru Watanabe, Shizuoka, JP;
Fumiaki Koizumi, Tokyo, JP;
Yuri Uehara, Tokyo, JP;
ON-CHIP BIOTECHNOLOGIES CO., LTD., Tokyo, JP;
SHIZUOKA PREFECTURE, Shizuoka, JP;
Abstract
The object of the present invention is to provide an evaluation method capable of accurately determining a metastasis of cancer, the stage of cancer progression, or the malignancy of cancer. The object can be solved by a method for detecting the degree of malignancy of each of the circulating tumor cells, characterized by the following steps: (a) bringing an epithelial cell-binding component, which specifically binds to a marker molecule expressed on epithelial cells and is fluorescently-labeled or luminescent enzyme-labeled, and a mesenchymal cell-binding component, which specifically binds to a marker molecule expressed on mesenchymal cells and is fluorescently-labeled or luminescent enzyme-labeled, into contact with a sample that possibly contains circulating tumor cells, (b) detecting a fluorescence signal or a luminescence signal of the epithelial cell-binding component and a fluorescence signal or a luminescence signal of the mesenchymal cell-binding component of each of the cells, and (c) determining the degree of epithelial-mesenchymal transition of circulating tumor cells based on the signal amount of the epithelial cell-binding component (E) and the signal amount of the mesenchymal cell-binding component (M).