The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 22, 2022
Filed:
Aug. 12, 2020
Roche Sequencing Solutions, Inc., Pleasanton, CA (US);
Thomas Albert, Danville, CA (US);
Frank Bergmann, Iffeldorf, DE;
Victor Lyamichev, Madison, WI (US);
Mohamed Yosry Hassan Mohamed, Munich, DE;
Tobias Oelschlaegel, Munich, DE;
Jigar Patel, Verona, WI (US);
Michael Schraeml, Penzberg, DE;
Wojtek Steffen, Murnau, DE;
Thomas Streidl, Seeshaupt, DE;
Roche Sequencing Solutions, Inc., Pleasanton, CA (US);
Abstract
In one aspect, the present disclosure provides a system and method for the identification and characterization of a transglutaminase. Further, the present disclosure provides transglutaminase enzymes for forming isopeptide bonds, methods of forming isopeptide bonds in the presence of transglutaminases, and substrate tags for use with transglutaminases. In another aspect, the present disclosure provides glutamine-containing substrates (or Q-tag substrates) that are more resistant to proteases/clipping and therefore, more stable, than other Q-tag substrates, and their uses in substrate tags for cross-linking to an amine-donor tag via an isopeptide bond mediated by a microbial transglutaminase.