The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 22, 2022
Filed:
Dec. 01, 2015
The Broad Institute, Inc., Cambridge, MA (US);
Baylor College of Medicine, Houston, TX (US);
Erez Lieberman-Aiden, Houston, TX (US);
Suhas Rao, Medfield, MA (US);
Elena Stamenova, Reading, MA (US);
Olga Dudchenko, Houston, TX (US);
Eric Lander, Cambridge, MA (US);
THE BROAD INSTITUTE, INC., Cambridge, MA (US);
BAYLOR COLLEGE OF MEDICINE, Houston, TX (US);
Abstract
Disclosed is an in situ method for detecting spatial proximity relationships between nucleic acid sequences, such as DNA, in a cell. The method includes: providing a sample of one or more cells comprising nucleic acids; fragmenting the nucleic acids present in the cells that leaves 5' overhanging ends; filling in the overhanging ends with at least one labeled nucleotide; joining the filled in end of the fragmented nucleic acids that are in close physical proximity to create one or more end joined nucleic acid fragments having a junction; isolating the one or more end joined nucleic acid fragments using the labeled nucleotide; and determining the sequence at the junction of the one or more end joined nucleic acid fragments.