The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 22, 2022
Filed:
May. 27, 2021
Zymergen Inc., Emeryville, CA (US);
Stephen Blaskowski, Oakland, CA (US);
Sara da Luz Areosa Cleto, Emeryville, CA (US);
Cameron Coates, Oakland, CA (US);
Aaron Miller, Berkeley, CA (US);
Sharon Nademanee, Alameda, CA (US);
Melissa Netwal, Oakland, CA (US);
Kedar Patel, Fremont, CA (US);
Shawn Szyjka, Martinez, CA (US);
Philip Weyman, Alameda, CA (US);
Solomon Henry Stonebloom, Alameda, CA (US);
Colin Scott Maxwell, Emeryville, CA (US);
Elizabeth Lauren Meier, Oakland, CA (US);
Zymergen Inc., Emeryville, CA (US);
Abstract
The present invention relates to methods for editing the genome of a microbial host cell in successive rounds of transformation. The method allows the introduction of genetic edits into the genome of a microbial host cell in an iterative fashion that does not require the use of functional counterselection following at least one round of transformation. It can be used to rapidly stack genetic edits in the genome of a microbial host cell. Kits for performing the methods are also disclosed.