The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 15, 2022
Filed:
Sep. 30, 2019
Government of the United States, As Represented BY the Secretary of the Air Force, Wright-Patterson AFB, OH (US);
Joseph M Slocik, Dayton, OH (US);
Rajesh R. Naik, Centerville, OH (US);
Patrick B Dennis, Cincinnati, OH (US);
United States of America as represented by the Secretary of the Air Force, Wright-Patterson AFB, OH (US);
Abstract
A method comprises the steps of: providing aqueous enzymes, wherein the enzymes are one of BamH1, EcoR1, EcoR2, and EcoRV; titrating the aqueous enzymes with a mixture of small molecule anions to form an enzyme/anion pair solution. Small molecule anions may comprise one or more of D- and L-amino acid esters, small D- and L-peptide pairs, and DL lactate solution. The titrating step is performed until the enzyme/anion pair solution becomes negative by zeta potential measurement. The at least one enzyme/anion pair may be dialyzed to remove excess anionic polymer using a dialysis membrane. The enzyme/anion pair solution is lyophilized to remove all of the water, forming a lyophilized solid of ultra-stable enzymes. Before titration, the positive electrostatic charge of the aqueous enzymes may be confirmed by measuring a positive zeta potential value.