The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 15, 2022

Filed:

Aug. 06, 2014
Applicants:

Riken, Wako, JP;

Sumitomo Chemical Company, Limited, Tokyo, JP;

Inventors:

Yoshiki Sasai, Kobe, JP;

Chikafumi Ozone, Wako, JP;

Yuko Maruyama, Wako, JP;

Assignees:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 5/079 (2010.01);
U.S. Cl.
CPC ...
C12N 5/0621 (2013.01); C12N 2500/90 (2013.01); C12N 2501/115 (2013.01); C12N 2501/155 (2013.01); C12N 2501/727 (2013.01); C12N 2506/02 (2013.01);
Abstract

The present invention provides a production method of a cell aggregate containing an anterior eye segment tissue or a partial structure thereof, or a precursor tissue thereof, including culturing pluripotent stem cell aggregates in suspension in the presence of a bone morphogenic factor signal transduction pathway activating substance to induce self-organization of an anterior eye segment tissue or a partial structure thereof, or a precursor tissue thereof. In one embodiment, the bone morphogenic factor signal transduction pathway activating substance is BMP4. In one embodiment, the suspension culture is performed entirely or partially in the presence of a fibroblast growth factor. The produced cell aggregate can further contain a neural retinal tissue.


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