The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 15, 2022

Filed:

Nov. 15, 2019
Applicant:

Precigenome, Llc, San Jose, CA (US);

Inventors:

Chen Li, Sunnyvale, CA (US);

Yunfeng Ling, San Jose, CA (US);

Cifeng Fang, Redmond, WA (US);

Yaqi Wang, San Jose, CA (US);

Yu Liu, San Jose, CA (US);

Assignee:

Precigenome, LLC, San Jose, CA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
B01L 3/00 (2006.01); C12Q 1/68 (2018.01); C12M 1/00 (2006.01); C12P 19/34 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/6844 (2018.01); C12Q 1/686 (2018.01); G01N 21/64 (2006.01);
U.S. Cl.
CPC ...
B01L 3/502715 (2013.01); B01L 3/50273 (2013.01); B01L 3/502707 (2013.01); G01N 21/645 (2013.01); G01N 21/6486 (2013.01); B01L 2200/10 (2013.01); B01L 2200/12 (2013.01); B01L 2200/142 (2013.01); B01L 2300/0654 (2013.01); B01L 2300/1805 (2013.01); B01L 2400/0487 (2013.01); C12Q 1/6844 (2013.01); G01N 2021/6471 (2013.01);
Abstract

Disclosed herein are microfluidic devices and systems for amplifying and detecting a target polynucleotide, comprising: one or more wells for receiving one or more substrates; a droplet generation channel in fluid communication with the one or more wells, wherein the microfluidic channel is adapted to generate droplets; and a chamber in fluid communication with the droplet generation channel, and adapted to collect droplets generated by the droplet generation channel, and further adapted to perform nucleic acid amplification in droplets, and further adapted to detect light signal from droplets. Also disclosed are methods of using the same.


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