The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 08, 2022

Filed:

Aug. 09, 2018
Applicant:

Imec Vzw, Leuven, BE;

Inventors:

Paolo Fiorini, Brussels, BE;

Tim Stakenborg, Leuven, BE;

Frederik Colle, Linden, BE;

Assignee:

IMEC VZW, Leuven, BE;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/686 (2018.01); B01L 3/00 (2006.01); B01L 7/00 (2006.01);
U.S. Cl.
CPC ...
B01L 3/502784 (2013.01); B01L 7/52 (2013.01); C12Q 1/686 (2013.01); B01L 2200/061 (2013.01); B01L 2200/0673 (2013.01); B01L 2200/10 (2013.01); B01L 2300/0627 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0867 (2013.01); B01L 2300/0883 (2013.01); B01L 2300/1827 (2013.01);
Abstract

A micro-fluidic devicefor performing digital PCR is presented. The device comprises: a semiconductor substrate; a first micro-fluidic channel, comprising an inletand an outlet, embedded in the semiconductor substrate; a heating elementthermally coupled to the first micro-fluidic channel; a droplet generatorconnected to the inletof the first micro-fluidic channelfor generating droplets and pumping generated droplets at a flow rate into the first micro-fluidic channel; characterized in that: the heating elementis a single heating element connected to a temperature control unitconfigured to cycle the temperature of the complete first micro-fluidic channelthrough at least two temperature values; and wherein the flow rate of the droplet generatoris adaptable. Further, a method to perform digital PCR is presented using the micro-fluidic device


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