The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 01, 2022

Filed:

Feb. 05, 2020
Applicant:

Oxford Nanopore Technologies Ltd., Oxford, GB;

Inventors:

Clive Gavin Brown, Cambridge, GB;

James Anthony Clarke, Oxford, GB;

Graham Hall, Oxford, GB;

Gavin Harper, Sonning, GB;

Andrew John Heron, Oxford, GB;

James White, Oxford, GB;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6869 (2018.01); G01N 27/447 (2006.01); G01N 33/487 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6869 (2013.01); G01N 27/44717 (2013.01); G01N 27/44791 (2013.01); G01N 33/48721 (2013.01);
Abstract

The claimed invention relates to a method of processing a polynucleotide, by obtaining a sense polynucleotide strand comprising a homopolymeric region that is longer that the reading section of a nanopore; synthesizing an antisense polynucleotide strand under conditions in which a nucleotide analog is incorporated at random in a reverse complement of the homopolymer region, such that the length of the homopolymer region in the antisense polynucleotide strand is shorter than the reading section of the nanopore; and moving the antisense polynucleotide strand through the nanopore such that a proportion of the antisense polynucleotide strand interacts with the nanopore.


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