The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 01, 2022

Filed:

Oct. 19, 2017
Applicant:

Q-linea Ab, Uppsala, SE;

Inventors:

Markus Klintstedt, Uppsala, SE;

Harer Osman, Uppsala, SE;

Mats Gullberg, Sollentuna, SE;

Assignee:

Q-LINEA AB, Uppsala, SE;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/24 (2006.01); C12M 1/40 (2006.01); C12M 1/26 (2006.01); G01N 1/40 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/24 (2013.01); C12M 21/18 (2013.01); C12M 33/14 (2013.01); G01N 1/4005 (2013.01); G01N 1/4077 (2013.01); G01N 2001/4016 (2013.01); G01N 2001/4088 (2013.01);
Abstract

The present invention provides a method of recovering viable microbial cells from a complex sample, said method comprising: a) providing a sample having a volume of at least 1 ml; b) contacting said sample with a buffer solution and one or more proteases, wherein said buffer solution has a pH of at least pH 6 and less than pH 11, wherein said buffer solution and said one more proteases do not comprise a detergent or a chaotrope, and wherein the buffer solution/protease/sample mixture is non-hypotonic; c) filtering the mixture obtained in step (b) through a filter suitable for retaining microbial cells; and d) recovering the microbial cells retained by the filter in step (c), wherein the recovered microbial cells are viable, and a microbial recovery device for the same.


Find Patent Forward Citations

Loading…