The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 01, 2022

Filed:

Apr. 23, 2018
Applicant:

Ucb Biopharma Srl, Brussels, BE;

Inventors:
Assignee:

UCB BIOPHARMA SRL, Brussels, BE;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 21/00 (2006.01); C12P 21/02 (2006.01); C12N 9/52 (2006.01); C07K 16/28 (2006.01); C12N 15/70 (2006.01);
U.S. Cl.
CPC ...
C12P 21/00 (2013.01); C07K 16/283 (2013.01); C12N 9/52 (2013.01); C12N 15/70 (2013.01); C12P 21/02 (2013.01); C07K 2317/10 (2013.01);
Abstract

The present disclosure relates to a recombinant gram-negative bacterial cell comprising: a) a mutant spr gene encoding a spr protein having a mutation at one or more amino acids selected from D133, H145, H157, N31, R62, I70, Q73, C94, S95, V98, Q99, R100, L108, Y115, V135, L136, G140, R144 and G147 and b) a gene capable of expressing or overexpressing one or more proteins capable of facilitating protein folding, such as FkpA, Skp, SurA, PPiA and PPiD, wherein the cell has reduced Tsp protein activity compared to a wild-type cell, methods employing the cells, use of the cells in the expression of proteins in particular antibodies, such as anti FcRn antibodies and proteins made by the methods described herein.


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