The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 01, 2022

Filed:

Sep. 11, 2015
Applicant:

Whitehead Institute for Biomedical Research, Cambridge, MA (US);

Inventors:

Susan L. Lindquist, Cambridge, MA (US);

Priyanka Narayan, Cambridge, MA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/81 (2006.01); C07K 14/775 (2006.01); C07K 14/47 (2006.01); G01N 33/92 (2006.01); C12Q 1/02 (2006.01); G01N 33/50 (2006.01); G01N 33/68 (2006.01); A61K 38/00 (2006.01);
U.S. Cl.
CPC ...
C12N 15/81 (2013.01); C07K 14/4711 (2013.01); C07K 14/775 (2013.01); C12Q 1/02 (2013.01); G01N 33/5014 (2013.01); G01N 33/5058 (2013.01); G01N 33/6896 (2013.01); G01N 33/92 (2013.01); A61K 38/00 (2013.01); C07K 2319/02 (2013.01); G01N 2333/775 (2013.01); G01N 2500/10 (2013.01); G01N 2800/2821 (2013.01); G01N 2800/50 (2013.01); G01N 2800/709 (2013.01);
Abstract

Disclosed are yeast cells expressing a polypeptide comprising a signal sequence and a human ApoE protein. In some embodiments the polypeptide comprises ApoE2. In some embodiments the polypeptide comprises ApoE3. In some embodiments the polypeptide comprises ApoE4. Also disclosed are methods of screening yeast cells to identify compounds that prevent or suppress Apo-induced toxicity. Compounds identified by such screens can be used to treat or prevent neurodegenerative disorders such as Alzheimer's disease. Also disclosed are methods of screening yeast cells to identify genetic suppressors or enhancers of ApoE-induced toxicity. Also disclosed are genetic suppressors or enhancers of ApoE-induced toxicity identified using the methods, and human homologs thereof. Also disclosed are methods of identifying compounds that modulate expression or activity of genetic modifiers of ApoE-induced toxicity.


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