The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 22, 2022

Filed:

Aug. 19, 2019
Applicant:

Illumina, Inc., San Diego, CA (US);

Inventors:

Min-Jui Richard Shen, Poway, CA (US);

Jonathan Mark Boutell, Nr Saffron Walden, GB;

Kathryn M. Stephens, Poway, CA (US);

Mostafa Ronaghi, San Diego, CA (US);

Kevin L. Gunderson, Encinitas, CA (US);

Bala Murali Venkatesan, San Diego, CA (US);

M. Shane Bowen, La Jolla, CA (US);

Kandaswamy Vijayan, San Diego, CA (US);

Assignee:

Illumina, Inc., San Diego, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6848 (2018.01); C12Q 1/6844 (2018.01); C12Q 1/6874 (2018.01); B01J 19/00 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6848 (2013.01); B01J 19/0046 (2013.01); C12Q 1/6844 (2013.01); C12Q 1/6874 (2013.01); B01J 2219/00585 (2013.01); B01J 2219/00587 (2013.01); B01J 2219/00608 (2013.01); B01J 2219/00621 (2013.01); B01J 2219/00637 (2013.01); B01J 2219/00653 (2013.01); B01J 2219/00675 (2013.01); B01J 2219/00722 (2013.01);
Abstract

A method including (a) providing an amplification reagent including an array of sites, and a solution having different target nucleic acids; and (b) reacting the amplification reagent to produce amplification sites each having a clonal population of amplicons from a target nucleic acid from the solution. The reacting can include simultaneously transporting the nucleic acids to the sites at an average transport rate, and amplifying the nucleic acids that transport to the sites at an average amplification rate, wherein the average amplification rate exceeds the average transport rate. The reacting can include producing a first amplicon from a nucleic acid that transports to each of the sites, and producing subsequent amplicons from the nucleic acid or from the first amplicon, wherein the average rate at which the subsequent amplicons are generated exceeds the average rate at which the first amplicon is generated.


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