The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 22, 2022
Filed:
Feb. 09, 2017
Applicant:
The Board of Trustees of the Leland Stanford Junior University, Stanford, CA (US);
Inventors:
Karl A. Deisseroth, Stanford, CA (US);
Emily L. Sylwestrak, Redwood City, CA (US);
Priya Rajasethupathy, Stanford, CA (US);
Matthew Wright, Mountain View, CA (US);
Assignee:
The Board of Trustees of the Leland Stanford Junior University, Stanford, CA (US);
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6841 (2018.01); C12Q 1/6806 (2018.01); G02B 21/06 (2006.01); C12Q 1/6837 (2018.01); G01N 1/30 (2006.01); C12Q 1/68 (2018.01); G02B 21/00 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6841 (2013.01); C12Q 1/68 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6837 (2013.01); G01N 1/30 (2013.01); G02B 21/06 (2013.01); G01N 2001/305 (2013.01); G02B 21/0024 (2013.01);
Abstract
Methods, kits, and systems for fixation of RNA permitting its detection in intact tissue, such as, large volume of mammalian tissue are disclosed. The methods, kits, and systems utilize carbodiimide-based chemistry to stably retain RNAs in tissue clarified using CLARITY. Also provided herein are methods, kits, and systems for detection of RNAs in clarified tissue.