The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 22, 2022
Filed:
Mar. 13, 2017
Applicant:
Fresenius Kabi Deutschland Gmbh, Bad Homburg, DE;
Inventors:
Matthieu Stettler, Vucherens, CH;
Hervé Broly, Chatel-St Denis, CH;
Thomas Solacroup, Blonay, CH;
Yolande Rouiller, Lausanne, CH;
Assignee:
FRESENIUS KABI DEUTSCHLAND GMBH, Bad Homburg, DE;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07K 16/14 (2006.01); C12P 21/02 (2006.01); C07K 16/24 (2006.01);
U.S. Cl.
CPC ...
C12P 21/02 (2013.01); C07K 16/241 (2013.01); C07K 2317/14 (2013.01); C07K 2317/21 (2013.01);
Abstract
The present invention relates to a method of maintaining cell viability at a level of at least 80% for up to 14 days in a fed-batch culture of cells producing a recombinant protein, the method comprising maintaining the cell culture medium at a pH of 6.5 to 7.0 and at a temperature of 31° C. to 35° C. for at least 5 consecutive days of the duration of the cell culture method.