The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 22, 2022
Filed:
Jan. 13, 2017
Applicant:
Astrazeneca Ab, Södertälje, SE;
Inventors:
Marcello Maresca, Södertälje, SE;
Amir Taheri-Ghahfarokhi, Södertälje, SE;
Mohammad Bohlooly-Yeganeh, Södertälje, SE;
Lorenz M. Mayr, Södertälje, SE;
Assignee:
ASTRAZENECA AB, Södertälje, SE;
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/11 (2006.01); C12N 15/85 (2006.01); C12N 15/87 (2006.01); C12N 15/90 (2006.01); C12N 5/074 (2010.01); C12N 15/10 (2006.01); C12N 15/63 (2006.01); C12N 5/0735 (2010.01);
U.S. Cl.
CPC ...
C12N 15/102 (2013.01); C12N 5/0606 (2013.01); C12N 5/0696 (2013.01); C12N 15/111 (2013.01); C12N 15/63 (2013.01); C12N 15/85 (2013.01); C12N 15/87 (2013.01); C12N 15/907 (2013.01); C12N 2310/20 (2017.05); C12N 2320/10 (2013.01); C12N 2320/12 (2013.01);
Abstract
Provided herein, in some embodiments, are nucleic acid-based tools that may be used for high-throughput functional genomics studies as well as for the generation of knockout (gene inactivation or deletion) or knockin (gene activation or insertion) cell lines. Tools of the present disclosure include an 'activatable reporter cassette,' a guide RNA construct and a nuclease that can be used together, for example, to modify and isolate targeted cells of interest.