The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 22, 2022

Filed:

Nov. 21, 2012
Applicant:

Toray Industries, Inc., Tokyo-to, JP;

Inventors:

Hiroyuki Kurihara, Kamakura, JP;

Katsushige Yamada, Kamakura, JP;

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
B01D 61/18 (2006.01); C12M 1/00 (2006.01); C12P 21/00 (2006.01); B01D 61/16 (2006.01); B01D 61/14 (2006.01); C12N 9/42 (2006.01);
U.S. Cl.
CPC ...
B01D 61/18 (2013.01); B01D 61/142 (2013.01); B01D 61/145 (2013.01); B01D 61/16 (2013.01); C12M 21/14 (2013.01); C12M 47/10 (2013.01); C12N 9/2437 (2013.01); C12P 21/00 (2013.01); B01D 2311/2688 (2013.01); B01D 2317/025 (2013.01); B01D 2317/027 (2013.01);
Abstract

A method of producing cellulase includes steps (1) to (3): (1) subjecting an aqueous solution of cellulase derived from filamentous fungi to filtration through an ultrafiltration membrane with a molecular weight cut off of 100,000 to 200,000 to obtain a filtrate and concurrently obtain a concentrated enzyme liquid as a retentate; (2) further subjecting the filtrate obtained in step (1) to filtration through a second ultrafiltration membrane with a molecular weight cut off of 5,000 to 50,000 to obtain a second concentrated enzyme liquid as a retentate; and (3) mixing the concentrated enzyme liquid obtained in steps (1) and (2) to obtain cellulase derived from filamentous fungi.


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