The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 15, 2022
Filed:
Mar. 28, 2016
Nanjing Annoroad Gene Technology Co. Ltd, Nanjing, CN;
Annoroad Gene Technology (Beijing) Co., Ltd, Beijing, CN;
Xiuli Wang, Beijing, CN;
Wang Wang, Beijing, CN;
Ruilin Jing, Beijing, CN;
Zhaoling Xuan, Beijing, CN;
Dawei Li, Beijing, CN;
Junbin Liang, Beijing, CN;
Chongjian Chen, Beijing, CN;
NANJING ANNOROAD GENE TECHNOLOGY CO. LTD, Beijing, CN;
ANNOROAD GENE TECHNOLOGY (BEIJING) CO., LTD, Beijing, CN;
Abstract
The present invention provides a method for constructing a next-generation sequencing library for detecting low-frequency mutations, and a kit thereof. The constructing method comprises steps of obtaining blunt-end DNA fragments, obtaining DNA fragments with A-tail at the 3' end, obtaining adapter-added DNA fragments using a specific nucleotide sequence and obtaining amplification products using a specific nucleotide sequence.