The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 15, 2022

Filed:

Dec. 07, 2018
Applicant:

Kaneka Corporation, Osaka, JP;

Inventors:

Hiroshi Kita, Hyogo, JP;

Toshihiro Shikakura, Hyogo, JP;

Hirofumi Maeda, Hyogo, JP;

Keishi Takatsu, Hyogo, JP;

Assignee:

KANEKA CORPORATION, Osaka, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/10 (2006.01); C12P 21/00 (2006.01); C12N 15/09 (2006.01); C07K 2/00 (2006.01); C40B 40/10 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1041 (2013.01); C07K 2/00 (2013.01); C12N 15/09 (2013.01); C12P 21/00 (2013.01); C40B 40/10 (2013.01);
Abstract

A method for producing a ribosome display complex includes obtaining a ribosome complex including an unmodified polypeptide chain, an mRNA molecule and a ribosome by initiating translation of the mRNA molecule in a cell-free peptide synthesis system including the ribosome, and modifying the unmodified polypeptide chain by reacting a side chain reactive functional group in the unmodified polypeptide chain with a modifying reagent to produce a ribosome display complex including a modified polypeptide chain, the mRNA molecule and the ribosome. The unmodified polypeptide chain includes at least one reactive amino acid residue selected from the group consisting of a cysteine residue, a lysine residue, a histidine residue and a tryptophan residue. The at least one reactive amino acid residue includes the side chain reactive functional group, and the mRNA molecule includes a base sequence encoding an amino acid sequence of the polypeptide chain.


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